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(1) Abbreviations for restriction enzyme sites: H, Hin dIII.

(2) A single band was found for each restriction enzymes and combinations mentioned above.

(3) The Restriction Enzyme Database from NEB.

(4) Restriction enzyme map simulated by pDraw32 software is consistent basically with the previous report.

(5) There were restriction enzyme sites of Smaand Sdu in UCP 3 gene of pigs.

(6) Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.

(7) Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid.

(8) Methods Allele-specific restriction enzyme was used to analyze patient, her mother and brother and 40 healthy volunteers.

(9) And have been identified by PCR and restriction enzyme digestion successfully constructed recombinant viruses. Sentencedict.com

(10) Restriction enzyme:Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.

(11) Results:After being identified by PCR, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.

(12) Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes.

(13) METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.

(14) By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.

(15) Control amplifications on samples to show that a constant restriction enzyme site can be cut strengthen confidence in the analysis.

(16) Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.

(17) To improve the discrimination power of AFLP for peanut cultivars of similar origin, different restriction enzyme combinations and more primer pairs may be needed.

(18) The recombinant plasmid pcDNA3 F was stable within the host stain ZJ111 in vitro and in vivo as shown by restriction enzyme analysis and PCR identification of the F gene.

(19) The restriction digestion system consisted of 10μ L PCR products, 2.0μ L 10× Buffer[sentencedict.com], 83.350 nkat restriction enzyme and 7.5μ L ddH 2 O.

(20) It enables a specific gene to be located on a particular restriction enzyme fragment.

(21) Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.

(22) The mutation was confirmed by digestion of the PCR products with restriction enzyme.

(23) The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restriction enzyme analysis.

(24) CONCLUSION:Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.

(25) Finally a recombinant plasmid named pcNcSRS2, which was identified by PCR, restriction enzyme analysis and sequencing, was obtained.

(26) Methods Isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method.

(27) Positive clones of F gene were identified by PCR and restriction enzyme digestion and then sequenced.

(28) Methods: The serum samples were collected from 56 patients with morbilliform maculopapular eruption. The target DNA of CBV was detected by RT-PCR and restriction enzyme analysis.